1) The filler retains the title compound
Solid phase extraction operation generally has four steps:
Activate - remove impurities from the column and create a solvent environment.
Loading - the sample is dissolved with a certain solvent, transferred into the column and the components remain on the column.
Leaching - to maximize the removal of disturbances.
Elution - elutes the test substance with a small volume of solvent and collects it.
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1. Compared with the traditional liquid-liquid extraction and protein precipitation method, solid-phase extraction has unparalleled advantages:
Purification method: LLE (liquid-liquid extraction)
Advantages: No special equipment and materials required
Disadvantages: 1. Operation cumbersome and time-consuming
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Solid Phase Extraction (SPE) is a sample pretreatment technology developed from the mid-1980s. By liquid-solid extraction and liquid chromatography technology developed from the combination. The separation, purification and enrichment of the samples were realized by selective adsorption and desorption of the sample components through solid phase packing. The main purpose is to reduce the sample matrix interference, improve the detection sensitivity.
The basic principle and method of solid phase extraction:
SPE is based on the theory of liquid-solid phase chromatography, which is a kind of physical extraction process including liquid phase and solid phase by means of selective adsorption and selective elution. The sample can also be enriched, separated and purified. As a simple chromatographic process.
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1. Sample extraction
Accurately weighed shower gel 0.2 g (accurate to 0.01 g) in 10 mL stoppered plastic centrifuge tube, add 3 mL of saturated sodium chloride solution, vortex oscillation 1 min to disperse the sample, accurately add 2 mL of acetonitrile, Spin the extraction of 2 min, 5000 r / min centrifugal 10 min, aspiration of the supernatant in another 50 mL stoppered plastic centrifuge tube, the lower sodium chloride solution with 2 mL of acetonitrile repeated extraction step once, combined with the second acetonitrile extract, 40 mL of high purity water was added to the extract, mixed with 0.2 mL of 10% potassium ferrocyanide solution, mixed with 0.2 mL of 20% aqueous solution of zinc acetate, centrifuged at 5000 r / min for 10 min, supernatant To be purified.
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