cableties的部落格

Pipette is common used lab consumables in every laboratory,the right using method will influence the correctness of samples volume of pipette,then show effect on the experiment result.So here are some tips for using the pipettes:

1. Adjustment Pipette range
In the regulation range, if you want to transfer from a large volume of small volume, according to the regulation method of normal, counterclockwise rotation knob; but if small volume adjustment is the large volume, may be the first clockwise rotation of the scale knob to scale beyond range, and then back to set the volume, so as to ensure the highest accuracy take the amount. In the process, do not turn the Pipette button out of the range, otherwise it will be stuck inside the mechanical device and damaged the Pipette.
2. Assembly of the Pipette head (nozzle)
In the gun head up the Pipette, the correct method is to move the gun into the gun head, a little bit of force around the rotation can make it close. If the utility model is a multi-channel (such as a 8 or 12) Pipette, the first gun of the Pipette can be aligned with the first gun head, and then is inserted obliquely. The gun head is tightly marked with a slightly more than O type ring, and can be seen in the connecting part to form a clear sealing ring. Three
3. Liquid (sample ) pipet method
Before moving the liquid, ensure that the Pipette, gun head and liquid are at the same temperature. When the liquid is absorbed, the Pipette is kept in a vertical state, and the gun head is inserted into the liquid surface under 2-3 mm. Before imbibition, the liquid can be sucked several times in order to moisten the suction nozzle (especially when it is necessary to absorb the viscous or different density of water). At this time can take two kinds of fluid transfer method.

1)Forward migration method. Press the button with the thumb to the first stop, then slowly release the button to return to the origin. Then the first stop button to pause for a moment to drain the liquid, press the button to stop second points blowing out the residual liquid. Final release button.
2)The reverse transfer method. This method is generally used for the transfer of high viscosity liquid, liquid foaming liquid, biological activity or trace liquid, its principle is the first to take more than set the range of liquid transfer liquid when not blowing out the residual liquid. Press the button to the second stop, slowly release the button to the origin. Then the button is pressed to the first stop point to discharge the liquid with good range, and the holding button is kept at the first stop point (not to press down again). Four
4.Correct placement of Pipette
After use, can be hung in the vertical pipette gun, but be careful not to fall off. When the pipette gun head with liquid, do not pipette horizontally or upside down, so as to avoid the back flow of the liquid corrosion piston spring.
Starlab can supply Pipettes in multiple choice,please kindly visit our website for more product details.

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