Advanced Pipette Using Precautions

This article we’d like to share the advanced quality pipette using method and tips to make sure the global user can handle them well.Hope well method and tips can helps to make the experiment be higher efficiency.

(1) To draw the liquid must be slowly and slowly release the thumb when using the pipette, never allow a sudden release, to prevent the solution inhaled too fast and into the liquid within the plunger to cause leakage.
(2) In order to obtain a higher accuracy pipette, the suction nozzle to be a pre-aspirated sample solution, and then the official pipetting, because the absorption of serum protein solution or organic solvent, the tip of the inner wall will be a layer of "liquid film" The amount of small and produce errors.

(3) The concentration and viscosity of the liquid, will produce errors, in order to eliminate the error compensation, can be determined by the test, the amount of compensation can be used to adjust the reading knob to read the reading window to set.
(4) Can be used to calculate the balance of the weight of pure water and calculated by way of calculation, to correct the extractor, 1ml distilled water at 20 ℃ weight 0.9982g.
(5) The pipette repeatedly hit the tip to tighten the method is very undesirable, long-term operation will make the internal parts loose and damage the pipette.
(6) Do not move the liquid when the pipette is not equipped with pipette suction.
(7) When setting the pipette range, please note that the range should not be rotated out of the range within the range of the pipette, otherwise it will jam the mechanism and damage it. In the display window, Pipettes.
(8) Pipettes are strictly prohibited to absorb a strong volatile, strong corrosive liquids (such as concentrated acid, concentrated alkali, organic matter, etc.).
(9) It is forbidden to use a pipette to mix the liquid.
(10) Do not use a large range of pipettes to remove small volumes of liquid, so as not to affect the accuracy. Also, if you need to remove a large amount of liquid outside the range, use one pipette to operate.
(11) If you do not use it again, move the range of the pipette to the maximum, so that the spring is in a relaxed state to protect the spring.
(12) The best regular cleaning pipettes, you can use soapy water or 60% isopropyl alcohol, and then distilled water, dry naturally.
(13) Before the high temperature sterilization of pipette , to ensure that the pipette can adapt to high temperatures.
(14) Calibration can be done in a 20-25 degree environment by repeating several times of weighing the distilled water.
(15) To check whether the use of leakage phenomenon.
Method to absorb the liquid after hanging vertically placed a few seconds to see if the liquid surface is down. If there exist any leakage, the reasons for roughly the following aspects:
1.Whether the suction matched to the pipette;
2.Make sure Spring piston is normal;
3.If it is volatile liquid (many organic solvents are so), it may be saturated vapor pressure problem. You can first suck a few times the liquid and then pipetting.
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