(1) mobile phase: must be used after chromatography through the filter after use, and the mobile phase ratio can not be completely in accordance with the standard, such as: ① norfloxacin capsule mobile phase with 0.025mmol / L, phosphoric acid Amine to adjust the pH to 3.O ± 0.1) a B (87:13).
Determination of sample retention time is long, after the experiment changed (84:16), so that the retention time control in 4min or so. ② Cefazolin sodium for injection: dissolved in sodium citrate solution (1.33g, 1.12g) was dissolved in 1000ml of a half (88:12), changed to (80:20) Misong sodium phosphate injection: methanol-034 potassium dihydrogen phosphate (58:42) changed to (64:36). ④ Shuanghuanglian injection: methanol a glacial acetic acid water (48: 1: 52) into (6,1: 1: 36) above the improvement can be satisfied with the results, fast. Good accuracy.
(2) abnormal column pressure: Gui low: the bubble inside the column, with the syringe to the bubble out, the column pressure back to normal. Column height: internal obstruction problems occur. Treatment: The column was rinsed with 0.05 mmol / IH S0 standard solution for 0.5 h, then rinsed with water for 1 h, and finally rinsed with methanol. If the above methods can not make the column pressure back to normal, then consider whether the machine parts of the problem.
(3) the accuracy of external standard method: the accuracy of the external standard method related to the determination of the results, so the injection must be accurate results, the standard peak area RSD value should be less than 2. O. The general injector should not have bubbles present when extracting samples.
(4) When the test sample or standard peak pinning phenomenon, should check whether the phenomenon of collapse of the column occurred, if any, then re-column or change the new pillars.
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