Pipette is an indispensable tool for experimental operation. For the top experts, it is important to have the minimum error of the experiment and repeat the reproducibility. It is necessary to pay attention to the correct Pipette.
1. Affect factors for the accuracy of Pipettes
(1) temperature:
Room temperature is low, the hand temperature is high, making the air expansion, inhalation of cold solution often occurs error
(2)Air tightness:
The combination of the tips and Pipette body, as well as the Pipette between the long-term wear, resulting in error
(3)Suction rate:
Easy to cause the tip of the bubble and will pollute the tip
(4)The evaporation of reagents:
Volatile,the steam into the tip, the internal pressure increases, the results in the liquid out, the pressure becomes larger and produce errors.
Solution: repeated suction 4-5 times can be improved.
