cableties的部落格

Pipette is an indispensable tool for experimental operation. For the top experts, it is important to have the minimum error of the experiment and repeat the reproducibility. It is necessary to pay attention to the correct Pipette.

1. Affect factors for the accuracy of Pipettes
(1) temperature:
Room temperature is low, the hand temperature is high, making the air expansion, inhalation of cold solution often occurs error
(2)Air tightness:
The combination of the tips and Pipette body, as well as the Pipette between the long-term wear, resulting in error
(3)Suction rate:
Easy to cause the tip of the bubble and will pollute the tip
(4)The evaporation of reagents:
Volatile,the steam into the tip, the internal pressure increases, the results in the liquid out, the pressure becomes larger and produce errors.
Solution: repeated suction 4-5 times can be improved.

2. Using method
Replace the tip method: the most accurate.
(1) adjust the knob to the desired scale position, know-how: the first can be slightly more than some, and then turn to the scale. Attention to prohibit more than the maximum scale, to avoid human error.
(2) plug tip: aseptic operation, we should pay attention to the action quickly and correctly, can not touch the tip of the hand, the intensity of action to be consistent, to prohibit waste action. Ordinary operation, the thumb can be used to tighten the thumb, but the hand can not touch tip tip.
(3) use finger to press the first paragraph, tip contact reagent. White tip into the liquid 1 mm, yellow tip 2-3 mm, blue tip2-4 mm, to avoid the impact of hydraulic errors.
(4) slow suction reagent, prohibit the speed too fast, the liquid into the tip.
(5) out of the tip, the first in the container wall gently against the tip of the liquid flow to the tip.
(7) tip top in the container wall slightly slide, keep a small amount of liquid tip, remove the target container, fingers remove the tip, be careful not to spill the reagent.

Starlab Tips Info:
Package of 96pcs/rack or 1000pcs/bag
Volume of 5ul,10ul,200ul,1000ul etc.
RNase/DNase/Pyrogen free
Related products: Standard Pipette Series ,Advanced Pipette Series ,Advanced Pipette Plus Series