
Pipette is common used lab consumables in every laboratory,the right using method will influence the correctness of samples volume of pipette,then show effect on the experiment result.So here are some tips for using the pipettes:
1. Adjustment Pipette range
In the regulation range, if you want to transfer from a large volume of small volume, according to the regulation method of normal, counterclockwise rotation knob; but if small volume adjustment is the large volume, may be the first clockwise rotation of the scale knob to scale beyond range, and then back to set the volume, so as to ensure the highest accuracy take the amount. In the process, do not turn the Pipette button out of the range, otherwise it will be stuck inside the mechanical device and damaged the Pipette.
2. Assembly of the Pipette head (nozzle)
In the gun head up the Pipette, the correct method is to move the gun into the gun head, a little bit of force around the rotation can make it close. If the utility model is a multi-channel (such as a 8 or 12) Pipette, the first gun of the Pipette can be aligned with the first gun head, and then is inserted obliquely. The gun head is tightly marked with a slightly more than O type ring, and can be seen in the connecting part to form a clear sealing ring. Three
3. Liquid (sample ) pipet method
Before moving the liquid, ensure that the Pipette, gun head and liquid are at the same temperature. When the liquid is absorbed, the Pipette is kept in a vertical state, and the gun head is inserted into the liquid surface under 2-3 mm. Before imbibition, the liquid can be sucked several times in order to moisten the suction nozzle (especially when it is necessary to absorb the viscous or different density of water). At this time can take two kinds of fluid transfer method.